3H-labelling of a synthetic decapeptide having LH and FSH releasing activity (LH-RH/FSH-RH)

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3 H-labelling of a synthetic decapeptide having LH and FSH releasing activity (LH-RH--FSH-RH).

A decapeptide capable to stimulate the release of LH and FSH from pituitary has been recently isolated from hypothalami of porcine origin and its amino acid sequence has been established as (Pyro)Glu-HisTrp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH, [ 1,2]. An identical decapeptide has been found in sheep hypothalami [ 3,4] . Several laboratories described syntheses of LH-RH/FSH-RH either by the solid pha...

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Effect of synthetic luteinizing hormone releasing hormone (LH/FSH-RH) in women with menstrual disorders.

Synthetic luteinizing hormone/follicle stimulating hormone-releasing hormone (LH/FSH-RH) (50 mug) was given intravenously to six women with oligomenorrhoea and to four women with secondary amenorrhoea. Peripheral venous blood was withdrawn at regular intervals over a 24-hour period. The concentrations of LH, FSH, and oestradiol-17beta were determined by radioimmunoassay. In all subjects there w...

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Response of serum LH, FSH and prolactin to injection of synthetic LH-RH into rat anterior pituitary.

Changes in serum LH, FSH and prolactin were measured by radioimmunoassay following injection of synthetic LH-RH into the anterior pituitary and median eminence of rats. The time course of serum levels of LH and FSH after injection of synthetic LH-RH into the pituitary showed a peak at 15 min. The synthetic LH-RH had a more marked releasing effect on LH than on FSH. After injection of 20 ng into...

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FSH LH : FSH ratio

Patient A was a 30-year-old white woman with a longstanding history of primary amenorrhea and infertility. By age 18 the patient had neither menstruated nor demonstrated evidence of normal breast development. At that time she was given oral contraceptives for four cycles and responded with normal vaginal bleeding. Laboratory evaluation at age 18 demonstrated a serum FSH (follicle-stimulating ho...

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Quick release of FSH stimulated by repeated applications of synthetic luteinizing hormone-releasing hormone (LH-RH) in immature male rats.

Serum FSH and LH levels were estimated by radioimmunoassay following single subcutaneous administration, and repeated subcutaneous administration at various intervals in immature male rats. Single injection of LH-RH induced slight increase in FSH levels in comparison with considerable increase in LH. By two successive injections, the release of FSH was markedly stimulated when the time interval...

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ژورنال

عنوان ژورنال: FEBS Letters

سال: 1972

ISSN: 0014-5793

DOI: 10.1016/0014-5793(72)80547-7